Ibicuruzwa
3-MorholilineOPONPOPONCOCICIC; CAS No: 1132-61-2
Synonyme: 3-Morholin-4-Ium-4-ylpropane-1-Sulfonate; Chebi: 39076; 3- (4-
Imiti yimiti ya 3-morpholineopropanekonic acide
● Kugaragara / ibara: ifu yera
Umuvuduko ukabije: 0pa saa 25 ℃
Gushonga Ingingo: 277-282 ºC
Indangagaciro iroba: 1.512
● PKA: 7.2 (kuri 25 ℃)
Flash Ingingo: 116 ºC
● Zab:75.22000
● Ubucucike: 1.298 G / CM3
● LogP: 0.61520
SHAKA TEMP.
.:H2O: m 1 kuri 20 ° C, Sobanura
● Amazi yoroheje. UKUNTU G / L (20 ºC)
● Xlogp3: -3.2
● Hydrogen Bond Abaterankunga kubara: 1
● Hydrogen Bond Kubara: 4
● Intebe ya Rotable Kubara: 3
● Misa nyayo: 209.07217913
Vera: Kubara Atom Biremereye: 13
Kugorana: 214
Amakuru ya hefy
● Pictogram (s):
Xi
Kode ya Hazard: xi
Imvugo: 36/37/38
● Amagambo y'umutekano: 26-36
Ingirakamaro
Inseko ya kanonical:C1COCC [NH +] 1Ccs (= O) (= O) [O-]
Ibisobanuro:Mopes (3-morpholilineropanekonic aside) ni buffer yatangijwe na nziza et al. mu myaka ya za 1960. Nibisakukirana kuri mes. Imiterere ya shimi irimo impeta ya morfoni. Iperie ni ph yuzuye pH ikubiyemo impeta ya piperazine. Hamwe na PKA ya 7.20, mope ni impende nziza ya sisitemu yibinyabuzima iri hafi-kutabogama ph..
Ikoresha:3. Dilut Fungal Inocolum.as buffer muri capillary-zone electrophores kugirango ikoreshwe .Kuko depores ya poroteyine kuva ku byitegererezo bya Algal. Mopes ikora nka Added Agent Agent yakoreshejwe mubushakashatsi butandukanye bwibinyabuzima. Mops yakoreshejwe nka: Ibigize Akagari Umuco Wakayili Kwinjira Byiza
Intangiriro irambuye
3-morpholineopropanesulfonic acide, uzwi kandi nka mops, ni igikoma cyimiti gisanzwe gikoreshwa nkumukozi wibanze mubisabwa bitandukanye nibinyabuzima. Ni ifu yera ya kirisiti ikundwa mumazi.
Mops ifite imiti yimiti ya C7H15No4s nuburemere bwa molekile ya 209.26 g / mol. Ifite agaciro ka PKA igera kuri 7.2, ikabigira buffer nziza yo kubungabunga ph hafi ya 7.0-7.5.
Kimwe mu gukoresha mops iri muri biologiya na molecular hamwe nubushakashatsi bwibinyabuzima kugirango bugenzure PH mubisohoka mubinyabuzima bitandukanye nubushakashatsi. Bikunze gukoreshwa mubisabwa nko kwigunga kwa ADN / RNA, gukuramo proteine, reaction yamamaza, na gel electrophoresis. Mops itanga kugenzura ihamye kandi ihoraho P P P PH, yemerera abashakashatsi gukomeza ibintu byiza kubigeragezo byabo.
Mops irashobora kandi gukoreshwa nka buffer mumuco wumuco kago no mubuhanga bwa chromatography. Bizwiho ultraviolet yo hasi (UV) ibyuma murwego rwa 240-300 Nm, bigatuma bikwiranye no gukoresha porogaramu ya UV ishingiye.
Iyo ukorana na mope, ni ngombwa kubyitwaramo neza. Bikwiye kubikwa ahantu hakonje, kwumye kandi birinda ibikoresho bidahuye. Ingamba z'umutekano, nko kwambara ibikoresho bikwiye, bigomba gukurikizwa mugihe ukemura iki kigo.
Kimwe n'imiti iyo ari yo yose, ni byiza gusubiramo urupapuro rwamakuru (SDD) hanyuma ugirire inama uwakoze cyangwa umwuga wujuje ibyangombwa kubikorwa byihariye no gukoresha amabwiriza yihariye.
Gusaba
3-MorholilineOPopanekonic Acide (mope) ifite porogaramu zitandukanye mumirima itandukanye. Porogaramu zimwe na zimwe zirimo:
Umukozi wa Buffring:Mops ikoreshwa kenshi nkumukozi wigenga mubinyabuzima nibinyabuzima. Ifasha kugumana ph ihamye mubisubizo, cyane cyane muri physiologiya ya 7.0-7.5
Ibinyabuzima bya MoleCumille na Biochissie: Mops ikoreshwa cyane nka buffer muri ADNALATION, RNA Gukuramo Proteine, Ibisubizo Byuzuye, na Gel electrophoresis. Itanga ibyangombwa bya PH kuri ibi bisuzumye kandi biremeza ibisubizo byizewe.
Umuco wa Akagari: Isoni ikoreshwa nkumukozi wibanze mumico ya selile. Ifasha kubungabunga PH murwego rwifuzwa, zitanga ibidukikije bihamye byo gukura kwa selile no kubaho.
Chromatography:Mops ikoreshwa nka buffer mubuhanga butandukanye bwa chromatography, harimo ion guhana chromatografi hamwe nubunini bwo guhezwa chromatografiya. Iremeza uko pH mugihe cyo gutandukana no kweza.
Synthesis cynthesis: Mops irashobora gukoreshwa nkicyifuzo cyangwa ukemuwe muri synthesique reactions. Guhagarara kwayo, kukecuruzwa, na ph bufferties bituma bikwiranye na porogaramu zitandukanye zitandukanye.
UV SPCTROSCOPY:Mops ifite ibyuma bike muri UV intera hagati ya 240-300 Nm, bigatuma ari ingirakamaro mubigeragezo bya UV. Iremera ibipimo nyabyo nta kwivanga kuri buffer ubwayo.
Izi nizo ngero nkeya za porogaramu nyinshi za acide 3-morpholilineropanerfonic (mope). Ibinyuranye na PH kugenzura PH bigira igikoresho cyingenzi muburyo butandukanye bwa siyanse nubushakashatsi.
