Ibicuruzwa
Intumisi; CES No:7365-45-9
Synonyme: 1- [4- (2-HYDROXYithyl) -1-Piperazinyl] ethane-2-sulfanicacid; 4- (2-hydroxyEyyl) aside); n- (2-hydroxyyerl) piperazine-n'-2-2-2-hyhasroxyworeyl); n ' aside); n'-2-hydroxyyerlpiperazine-n-2-ethaneskonic acide; TVZ 7; 1
Imiti yimiti ya hepes
● Kugaragara / Ibara: Ifu yera
Umuvuduko ukabije: 0pa saa 25 ℃
Gushonga Ingingo: 234-238 ° C.
Indangagaciro irohereza: N20 / D 1.339
Ingingo itetse: 408 ℃ [saa 101 325 pa]
● PKA: 7.5 (kuri 25 ℃)
● Zab:89.46000
● Ubucucike: 1.325 G / CM3
● LogP: -0.55930
● Ububiko Temp.:2-8 uzi
.:H2o: m 1 kuri 20 ° C, isobanutse, ibara
● Amazi yoroheje.:
● Xlogp3: -4.1
● Hydrogen Bond Abaterankunga kubara: 2
● Hydrogen Bond Kubara: 5
● Intebe ya Rotable Kubara: 4
● Misa nyayo: 238.09872823
Atom Atom Arem: 15
Kugorana: 254
Amakuru ya hefy
● Pictogram (s):
Xi
Kode ya Hazard: xi
Imvugo: 36/37/38
● Amagambo y'umutekano: 24 / 25-22-36-26
Ingirakamaro
Inseko ya kanonical:C1CN (CC [NH +] 1CCS (= o) (= o -]) cco
Ibisobanuro:Impesi yasobanuwe nkimwe mubyifuzo byiza byintego biboneka kubishakisha bibyara. Kuri ibinyabuzima Ph, molekile ni zwrityintic, kandi ikora neza nka buffer kuri ph 6.8 kugeza 8.2 (PKA 7.55). Mubisanzwe bikoreshwa mumico ya selile mugukora hagati ya 5mm kugeza 30 mm. Impesi yakoreshejwe muburyo butandukanye bwa porogaramu, harimo n'umuco wa tissue. Bikunze gukoreshwa mu guhuza imico ya selile mu kirere. Ihemu isanga imikoreshereze yayo muri vitro igerageza kuri mg.
Ikoresha:Uburozi muri selire. Ikoreshwa nka hydrogen ion buffer, ishobora kugenzura ph intera ndende ya PH mugihe kirekire. Kwibanda ni 10-50mmol / l. Mubisanzwe, 20mmol / shopepe mubisubizo byintungamubiri birashobora kubona ubushobozi bwa buffer. Buffer y'ibinyabuzima. Ifasha isanzwe ya siyanse y'ibinyabuzima, yakoreshejwe cyane cyane mu muco w'akagari kugira ngo akomeze ph. Ikora ibice byibice, bikoreshwa mugutegura buffers. Yasobanuwe nkimwe mubyifuzo byiza byintego biboneka kugirango ukoreshe mubushakashatsi bwibinyabuzima .Icyo cyakoreshejwe mu rwego rwo gutegura ibipimo bya kagoma.
Intangiriro irambuye
Uburapeihagaze kuri 2- (4- (2-hydroxyEyyl) piperazin-1-yl) aside Ethaneulfonic. Nubushakashatsi busanzwe bukoreshwa mubinyabuzima nubushakashatsi bwibinyabuzima. Ifasha ifasha gukomeza PH ihamye mu myuga y'umuco Imiterere yayo yimiti hamwe nimitungo bituma igira akamaro cyane mugukomeza ph ihoraho kandi nziza kubinyabuzima byinshi nibitekerezo. Ipepesiyo ikoreshwa mu muco w'akagari, kwezwa kwa poroteyine, na tekiniki za molekili.
Gusaba
Ubupfumu bufite ibyifuzo byinshi mubikorwa bitandukanye byubushakashatsi. Bimwe mubisabwa bikunze kugaragara ni:
Umuco w'akagari: Ipepesiyo ikunze gukoreshwa nkibice byo mu bitangazamakuru byumuco byakagari kugirango ukomeze ibidukikije bihamye PH. Ifasha kugenzura PH impinduka ziterwa nibikorwa bya metabolike no kwegeranya ibicuruzwa byimyanda mumico ngendanwa.
Enzyme Assay:Impesi ikoreshwa nkumukozi wigenga muri Enzyme asuzuma kugirango akomeze PH ahora, aricyo cyingenzi mubikorwa bikwiye, imikorere ikwiye. Ifasha kwemeza ibisubizo byukuri no guhindagurika.
Electrophoresis:Impesi ikoreshwa nkumukozi wamashanyarazi muri tekinike ya electrophondos nka polAcrylamide Gel electrophos (page) na agarose gel electrophores. Ifasha gukomeza PH yifuzwa kugirango atandukane neza no gusesengura Macronolecules nka proteyine na acide nucleic.
Kwezwa kwa proteine:Impesi rimwe na rimwe zikubiye mu bubiko bwo kwezwa kwa poroteyine kugirango ukomeze PH yifuzwa mugihe cyinzira zitandukanye zo kwezwa. Ifasha gukomeza umutekano nigikorwa cya poroteyine mugihe cyo kwezwa.
Ubuhanga bwa MoleCuming MoleCuming:Ubucukinguzi burashobora gukoreshwa muburyo butandukanye bwibinyabuzima bwa molecular, nka PCR (Polymeyese reaction) na ADN ikurikirana, kugirango bakomeze ph ihamye. Ifasha guhitamo imikorere ya enzymes no kureba ibisubizo byukuri kandi byizewe.
Ni ngombwa kumenya ko ibitekerezo byihariye byerekana kandi porogaramu yayo ishobora gutandukana bitewe nibibazo byubushakashatsi nibisabwa.
