Ibicuruzwa
HEPES umunyu wa sodium
Synonyme: HEPES umunyu wa sodiumi; 75277-39-3; HEPES umunyu wa hemisodium; 103404-87-1; Sodium 2- (4- ) piperazin-1-yl] Ethanesulfonate; HEPES (sodium); C8H17N2NaO4S; 4- (2-Hydroxyethyl) piperazine-1-Ethanesulfonic aside hemisodium umunyu; umunyu wa sodium ya etanesulfonike; Sodium 4- (2-hydroxyethyl) piperazin-1-ylethanesulphonate; UNII-Z9FTO91O8A; Z9FTO91O8A; sodium; 2- [4- 278-169-7; 1-Piperazineethanesulfonic aside, 4- (2-hydroxyethyl) -, umunyu wa sodiumi (1: 1); N- umunyu,> = 99.5% (titre); sodium hepes; N- (2-Hydroxyethyl) piperazine-N '- (2-etanesulfonic aside) umunyu wa sodium; MFCD00036463; HEPES, umunyu wa sodium; HEPES umunyu wa sodium, 98%; C8H18N2O4S.Na; GAHUNDA229142; YARI-14; CHEMBL3187284; DTXCID2024458; . icyiciro; AKOS015897769; AKOS015912229; AKOS015964204; NCGC00255791-01; CAS-75277-39-3; HY-108535; CS-0029103; FT-0610868; F20319; A838366; sodium N'-hydroxyethyl-N-pipe; BioXtra, > = 99.0%. -Hydroxyethyl) -1-piperazineethanesulfonic acide sodium umunyu; 4- (2-hydroxyethyl) -1-piperazineethanesulfonic aside, umunyu wa monosodium; piperazine-N- umunyu wa aside hemisodium; HEPES umunyu wa sodium, BioPerformance Yemejwe, ibereye umuco w'akagari, > = 99.5%
Umutungo wimiti wa HEPES Umunyu wa Sodium
Kugaragara / Ibara: ifu yera
Pressure Umuvuduko wumwuka: 0Pa kuri 25 ℃
Point Gushonga Ingingo: 234 ° C.
● PKA: 7.5 (kuri 25 ℃)
PSA：92.29000
Ens Ubucucike: 1.504 [kuri 20 ℃]
● LogP: -0.90190
Temp Ububiko bwububiko.: Ububiko kuri RT.
Yumva neza.: Hygroscopique
Ububasha.: H2O: 1 M kuri 20 ° C, bisobanutse, bitagira ibara
Ububasha bwamazi.:Burashonga mumazi.
● Abaterankunga ba Hydrogen Bond: 1
Kubara Amashanyarazi ya Hydrogen Kubara: 6
Kubara Impapuro zingana: 5
Mass Misa nyayo: 260.08067248
Count Kubara Atome Ikomeye: 16
● Ingorabahizi: 272
Amakuru Yizewe
● Pictogram (s):
Odes Kode ya Hazard:
Statement Ibisobanuro byumutekano: 22-24 / 25
Ni ingirakamaro
Amasomo ya Shimi:Ibindi Byakoreshejwe -> Ibinyabuzima
Urwenya rwa Canonical:C1CN (CCN1CCO) CCS (= O) (= O) [O -]. [Na +]
Ikoreshwa:HEPES sodium yumunyu ikwiranye nubushakashatsi bwumuco. Irashobora gukoreshwa mugutegura igisubizo cya buffer ya HEPES. Irashobora gukoreshwa nka buffer mugihe cya fosifora yerekana fibroblast. Irashobora gukoreshwa muguhimba dialyse igoye, ikoreshwa muguhitamo tiroxine yubusa muri serumu na radioimmunoassay. HEPES umunyu wa sodium yumuti urakwiriye gukoreshwa mubushakashatsi bwumuco. Irashobora gukoreshwa mugutegura igisubizo cya buffer ya HEPES. Irashobora gukoreshwa nka buffer mugihe cya fosifora yerekana muri fibroblast yemewe. Irashobora gukoreshwa muguhimba dialyse igoye, ikoreshwa muguhitamo tiroxine yubusa muri serumu na radioimmunoassay.
Intangiriro irambuye
HEPES umunyu wa sodium, bizwi kandi nka 4- (2-hydroxyethyl) piperazine-1-etanesulfonic acide sodium umunyu, nuburyo bukoreshwa bwa HEPES. Nibintu bya zwitterionic organic ikora nka buffering mubushakashatsi bwibinyabuzima na biohimiki.
HEPES umunyu wa sodium ukunze gukoreshwa mubitangazamakuru byumuco utugari hamwe na bffer biologique bitewe nubushobozi bwayo bwo gukomeza pH ihagaze neza mubihe byimiterere (pH 7.2 - 7.6). Irashobora gushonga cyane mumazi kandi ifite uburozi buke ugereranije, kuburyo bukoreshwa mubikorwa bitandukanye.
Ubwoko bwumunyu wa sodium ya HEPES bikundwa mubikorwa byinshi kuko byongera imbaraga kandi bigahinduka, ugereranije na acide yubusa. Iraboneka mubucuruzi nkifu ya kristaline yera ishobora gushonga byoroshye mumazi kugirango itegure ibisubizo byakazi.
Abashakashatsi bakunze gukoresha umunyu wa sodium ya HEPES mubuhanga bwa biyolojiya y’ibinyabuzima, umuco w’akagari, imisemburo ya enzyme, kweza poroteyine, n’ubundi bushakashatsi bwibinyabuzima aho kugenzura pH ari ngombwa. Ubushobozi bwacyo bwo guhuza no guhuza na sisitemu y'ibinyabuzima bituma ihitamo gukundwa no kubungabunga ibihe byiza kubikorwa no gutuza kwa molekile zitandukanye.
Mbere yo gukoresha umunyu wa sodium ya HEPES muburyo ubwo aribwo bwose, ni ngombwa gusubiramo ibitabo, kugisha inama ibyifuzo byabatanga, no guhuza ibitekerezo hamwe na pH ukurikije ibisabwa byihariye byubushakashatsi.
Gusaba
HEPES umunyu wa sodiumi usanga ikoreshwa cyane mubice bitandukanye byubushakashatsi bwibinyabuzima na biohimiki. Bimwe mubikorwa byingenzi bikoreshwa birimo:
Umuco w'akagari:HEPES umunyu wa sodium ukunze kwongerwaho mubitangazamakuru byumuco utugari kugirango ubungabunge pH ihamye murwego rwa physiologique kandi utange ibidukikije bikwiye kugirango ingirabuzimafatizo zikure kandi ziyongere.
Umukozi woherejwe:HEPES umunyu wa sodiumi ukoreshwa kenshi nka bffer muri biffer biologique hamwe nibisubizo, harimo enzyme enzyme, kweza poroteyine, hamwe nubuhanga bwa biologiya. Ifasha kugumana pH ihoraho mukurwanya impinduka za acide cyangwa alkaline.
Amashanyarazi: HEPES umunyu wa sodium ukoreshwa nka buffer muri gel electrophorei kugirango ugumane pH ihamye mugihe cyo gutandukanya acide nucleic cyangwa proteyine. Irinda guhinduka muri pH ishobora kugira ingaruka no kwimuka no gutandukana kwa molekile muri gel.
Intungamubiri za poroteyine: HEPES umunyu wa sodium wongeyeho ibisubizo bya poroteyine kugirango uhindure imiterere kandi ukomeze ibikorwa byabo. Ifasha kubungabunga ibidukikije byiza bya pH bikenewe kugirango protein ihagarare kandi ikore.
Igikorwa cya Enzyme: HEPES umunyu wa sodiumi ukoreshwa nka buffer mubitekerezo bitera imbaraga kugirango ubungabunge pH wifuza kubikorwa bya enzyme nziza. Ifasha kwemeza ko enzymes zikora neza kandi neza.
Kwerekana amashusho ya selile:HEPES umunyu wa sodium ukunze gushyirwa mubitangazamakuru byerekana amashusho kugirango bigerageze amashusho ya selile. Ubushobozi bwayo bufasha gufasha pH kwifuza kandi ikarinda ihindagurika rishobora kugira ingaruka kuri viabilite na fluorescence ya selile.
Ubuhanga bwibinyabuzima bwa molekuline: HEPES umunyu wa sodium ukoreshwa cyane muburyo butandukanye bwa biologiya ya biologiya, harimo ADN cyangwa RNA kwigunga, PCR, uko ADN ikurikirana, hamwe nisesengura rya poroteyine. Ubushobozi bwa buffering butanga ibisubizo nyabyo kandi byizewe muriki gikorwa.
Ni ngombwa kumenya ko uburyo bwihariye hamwe nubunini bwumunyu wa sodium ya HEPES bishobora gutandukana bitewe nibisabwa mubushakashatsi hamwe na sisitemu y'ibinyabuzima irimo kwigwa. Kubwibyo, nibyiza kugisha inama ibitabo hamwe nabatanga ibyifuzo kugirango bakoreshe neza.
