Ibicuruzwa
Hepps; cas No: 16052-06-5
Synonyme: Hepps; 16052-06-5; EPPROS; aside; 3- Aside; n-- (2-hydroxyyeryl) piperazine-n '(acide 3 ya propanexic); 4- (2-hydroxyerfonic; 240-198-8; BRN 3957385; 8JN5PQW9C; 4- (2-hydroxxiethyl) -1-hydroxxique sulfonic aside; ep1; n-2-hydroxyyerlpiperazine-n'-3-3-3-SPII-8JN5PQW9C; DTXSID30; COPS,> = 99.5% (tittion); HY-D0873; C9-H20-N2-O4-S; BFL0230; STL358 99%; AC-24630; AS-69273; EPPS, Bioxtra,> = 99.5% (tittion); LS-113260; CS-0015183; FT-0,20314; N- acide sulfonic; n-2-hydroxyyerlpiperazine-n-3-propaneulfonic aside; q1563525; w-107976; 3- (4- (2-hydroxxiethyl) -1-hydroxxiethyl) aside; (Hepps); 3-
Imiti yimiti ya hepps
● Kugaragara / Ibara: Ifu yera
Gushonga Ingingo: 237-239 ° C (Lit.)
Indangagaciro irohereza: 1.537
● PKA: 8.0 (kuri 25 ℃)
● Zab:89.46000
● Ubucucike: 1.285 G / CM3
● LogP: -0.16920
SHAKA TEMP.
.:H2o: m 1 kuri 20 ° C, isobanutse, ibara
● Amazi yoroheje. Arsonal mumazi. 25.2 G / L (20 ° C)
● Xlogp3: -3.6
● Hydrogen Bond Abaterankunga kubara: 2
● Hydrogen Bond Kubara: 6
● Intebe ya Rotable Kubara: 6
● Misa nyayo: 252.11437830
Atom Atom Arem: 16
Bigoye: 280
Amakuru ya hefy
● Pictogram (s):
Xi
Kode ya Hazard: xi
Imvugo: 36/37 / 38-22
● Amagambo y'umutekano: 24/9-36-26
Ingirakamaro
Amasomo ya Chimique:Azogen Ibice -> imiyoboro
Inseko ya kanonical:C1CN (CCN1CCs (= O) (= o) o) cco
Ikoresha:EPPS ni nziza ikoreshwa neza? Bikoreshwa kenshi nkumutandukanya muri ultrathin isoelectric yibanda kuri gels kandi yongerera imyanzuro ya fosifoglutase. Ikoreshwa nkumukozi wibanze muri biologiya na biokiesie.
Intangiriro irambuye
EPPS ihagaze kuri n- (2-hydroxyyeryl) piperazine-n '- acide 3-propanekonic). Numukozi wa ndofrionic agent akunze gukoreshwa mubinyabuzima nubushakashatsi bwibinyabuzima. Hano hari ingingo zingenzi zerekeye Epps:
Imitungo ya Buffer:EPPS ni umukozi mwiza ufasha kugumana ph ihoraho mubushakashatsi. Ifite agaciro ka PKA 8.09, bituma bikwiranye no kwivuza muri PH amanota 7.0-9.0.
Guhagarara:EPPS igaragaza umutekano mwiza hejuru yubushyuhe bwinshi kandi ntabwo ihinduka nubushyuhe bugereranije nabandi bakozi bugeraho nka Tris na FOSTFES. Uku gutumaho bituma bikwiranye no gukoresha mubushakashatsi bwibinyabuzima na biologiya bisaba kugenzurwa na PH.
Gusaba ibinyabuzima:EPPS ikoreshwa mubitabo bitandukanye bifatika, nkibisuku, Enzyme isuzuma, na ADN / RNA Gutwika RNA. Ubushobozi bwayo bukabije no gutuza kuri physiologiya Ph biko bifite agaciro muriyi porogaramu.
Umuco w'akagari:Epps irashobora kandi gukoreshwa nkumukozi wigenga mumisoro yumuco ka selile. Ifasha gukomeza ibidukikije bihamye PH bifasha gukura no kubungabunga ubwoko runaka.
Ions no guhuza:EPPS ni zwttarionic compound, bivuze ko irimo ibirego byiza kandi bibi. Iyi mikorere yemerera gutondeka neza aside hamwe nibisubizo byibanze. Birahuye na molekile zitandukanye za molekile kandi zizwiho kuba zifite ubuvange buke hamwe nibitekerezo byimikorere.
Iyo ukoresheje Epps, ni ngombwa gukurikiza amabwiriza yasabwe na PH yatanzwe nuwabikoze. Byongeye kandi, burigihe witonda hanyuma ukurikize protocole nziza yumutekano mugihe ukoresha imiti iyo ari yo yose.
Gusaba
Hepps (n-- (2-hydroxyyeryl) piperazine-n'-3-3-3-Propanekonic isanzwe ikunze gukoreshwa mubijyanye nubushakashatsi bwibinyabuzima nububiko bwibinyabuzima. Bimwe muri porogaramu zayo harimo:
Umuco w'akagari: Hepps ikoreshwa nkumukozi wibanze mumisoro yumuco selile kugirango agumane ph kandi itange ibidukikije bikwiranye no gukura kwakagari no kwibeshaho. Ifasha guhagarika PH muri physiologiya no kwirinda ingaruka mbi kubintu bya selile.
Enzyme Assay:Hepps ikunze gukoreshwa nka buffer muri enzyme isuzumye kugirango inoze ph isabwa kubikorwa byingenzi bya enzyme. Ubushobozi bwayo bufata bufasha gukomeza ph ihamye, kugenzura neza kandi byizewe enzyme.
Kwezwa kwa proteine:Hepps irashobora gukoreshwa nka buffer mubikorwa byo kwezwa kwa poroteyine. Ifasha gukomeza gushikama no mubikorwa bya poroteyine mugihe cyo kwezwa, nkinkingi chromatografiya cyangwa coromatografiya cyangwa kwerekana poroteatografiya cyangwa kwerekana poroteatografiya cyangwa kwerekana poroteatography cyangwa kwerekana poroteine ukoresheje tagi.
Electrophoresis:Hepps rimwe na rimwe ikoreshwa nka buffer muri Gel electrophores yo gutandukana no gusesengura poroteyine na acide ya nucleic. Ubushobozi bwa PH bufata ubushobozi bufasha gukomeza ph ihamye mugihe cyamateka ziruka, gufasha mugutandukanya neza molekile.
Ubuhanga bwa MoleCuming MoleCuming:Amashanyarazi arashobora gukoreshwa nka buffer muburyo butandukanye bwibinyabuzima, harimo na PCR (Polymeyese reaction), ADN iratandukanye, na ADN iratandukanye, hamwe nibitekerezo byimizine. Ubushobozi bwayo butunganijwe butuma ph ibintu byerekanwe muburyo bwo gutsinda kandi bunoze.
Ubushakashatsi burimo kuri proteins membrane:Hepps ikwiranye no kwiga poroteyine za membrane kubera imitungo yo hasi ya membrane. Bikoreshwa kenshi nka buffer mubushakashatsi burimo kuranga cyangwa kuvura proteins membrane.
Ni ngombwa kumenya ko kwibanda kuri keza na PH ya EPPPS bishobora gutandukana bitewe na porogaramu cyangwa igeragezwa. Kubwibyo, ni byiza kugisha inama ibitabo cyangwa gutanga ibyifuzo kugirango bikoreshe neza no kwibanda kubisige mubushakashatsi bwawe.
