Ibicuruzwa
Umunyu wa mopso sodium; CA No: 79803-73-9
Synonyme: 4-MorholinePropaNonicacid, B-Hydroxy-, Umunyu wa Monosodium (9CI)
Imiti yimiti ya mopso sodium umunyu
● PKA: 6.9 (kuri 25 ℃)
● Zab:98.28000
● LogP: -0.75660
SHAKA TEMP.
.:H2o: m 1 kuri 20 ° C, isobanutse, ibara
Amakuru ya hefy
● Pictogram (s):
Xi
Kode ya Hazard: xi
Imvugo: 36/37/38
● Amagambo y'umutekano: 26-36
Ingirakamaro
Ikoresha:Sodium ya mopso ni buffer y'ibinyabuzima nayo yitwaga nk'igisekuru cya kabiri "cyiza" cyiza cyerekana ko ushimishije ugereranije n'uburasirazuba "bwiza". PKA ya mopso sodium ni 6.9 zibigira umukandida mwiza wa buffer asaba phi munsi ya physiologie kugirango ukomeze ibidukikije bihamye. Sopium ya mopso ifatwa nkaho idasobanutse kumirongo ya selile ikagira ibisobanuro byinshi. Amaduka ashingiye kuri mopso yasobanuwe kugirango akosorwe kuri selile zingendo.
Intangiriro irambuye
Umunyu wa mopso sodium, uzwi kandi nka sodium 3- (n-morpholino) propaneulkonate, ni buffer isanzwe ikoreshwa mubinyabuzima nububiko. Ni ifu yera ya kirisiti ikundwa cyane mumazi. Umunyu wa mopso Sodium ukoreshwa nkibintu kugirango ukomeze agaciro ka PH uhamye mubisobanuro bitandukanye hamwe nibitekerezo byagaciro. Nibyingenzi cyane kubisabwa bisaba ph urwego rwa 6.5 kugeza 7.9 kubera agaciro ka PKA kagereranijwe ka 7.2. Iyi mbundi ya buffer ituma bikwiranye numuco wa seruki, kwezwa kwa proteine, na tekiniki za molekili.
Usibye ubushobozi bwayo, umunyu wa mopso sodium nanone ufite ubushobozi bwo guhagarika poroteyine zimwe na enzymes, zifasha gukomeza ibikorwa byabo n'imiterere. Bifatwa nkikineri ya Zwerionic, bivuze ko ishobora kubaho muburyo bwiza kandi bwishyurwa nabi, bitewe na PH yumuti. Iyo ukoresheje umunyu wa mopso sodium, ni ngombwa gupima no gutegura ibisubizo byubusa kugirango ugere ku rwego rwifuzwa. Ikimenyetso cya Phi Metero cyangwa PH kirasabwa kugirango ukurikirane kandi uhindure PH ukurikije.
Muri rusange, umunyu wa mopso sodium nigikoresho cyingenzi mubushakashatsi bwa laboratoire, gitanga ibidukikije bihamye no gushyigikira ubushakashatsi butandukanye nibinyabuzima.
Gusaba
Umunyu wa mopso sodium (3- (n-morholino) umunyu wa sodium wa sodium wa sodium) ufite porogaramu nyinshi zingirakamaro, cyane cyane murwego rwibinyabuzima na biotenology. Dore inzira zimwe umunyu wa mopso sodium ushobora kuba ingirakamaro:
Umukozi wa Buffring:Umunyu wa mopso Sodium ukoreshwa nkumukozi wigenga mubinyabuzima bitandukanye nibinyabuzima. Ifasha kugumana urwego ruhamye, aricyo gikorwa cyingenzi kubikorwa byiza byerekana, gushikama kwa proteyine, nibindi biologiya.
Proteine Crystallsation:Umunyu wa Mopso Sodium ukoreshwa cyane muri proteine Screstalisation ya ectstallisation kugirango yorohereze imikurire ya poroteyine yo hejuru. Ubushobozi bwayo buteye bufasha kubungabunga PH, ikenewe kugirango ituze n'imiterere ya kristu ya poroteyine.
Electrophoresis:Umunyu wa mopso Sodium ukora nkibice byamabuye mubuhanga nka sds-page (sodium dodecyl sulfate-polyacrylamide-polyacrylamide gel electrophoresis) ikoreshwa mugutandukanya no gusesengura poroteyine. Imitungo yayo ya buffer ifasha kubungabunga ph ihamye, iharanira gutandukana neza no gusesengura.
Enzyme Assay:Umunyu wa mopso sodium ni ingirakamaro muri enzyme assay na kinetic ubushakashatsi, aho bifasha gukomeza ibidukikije bihamye kandi bigenzurwa na PH. Ibi nibyingenzi kugirango dukorwe neza ibikorwa bya enzyme no gusobanukirwa ibipimo bya kinetic.
Ibinyabuzima Ibinyabuzima:Umunyu wa mopso Sodium ukoreshwa mu gushyiraho ibisubizo bya biokimiki, nka buffers yo gukuramo poroteyi, kwezwa, no kubika. Ubushobozi bwayo bukabije bwemeza ko PH ikomeza guhora, kugabanya itandukaniro mubintu bya poroteyine nibikorwa.
Ni ngombwa kumenya ko ibintu byihariye byihariye byo gukoresha umunyu wa mopso sodium birashobora gutandukana bitewe nubushakashatsi bwihariye cyangwa gusaba. Buri gihe reba protocole nkurikije amabwiriza yo kwitegura neza no gukoresha umunyu wa mopso sodium umunyu wa mopso sodium mubushakashatsi bwawe.
