Ibicuruzwa
Imiyoboro; CYA No: 5625-37-6
Synonyme:Acide 1,4-piperaziineiethane sulfonic;
1,4-piperaziineiethaneyphonic;
1,4-piperazineethanesUlfontate; piperazine-n, n, nis (2-ethanesulfonic);
Piperazine-n, N'-Bis (aside 2-ethaneskonic), umunyu wa sodium; imiyoboro
Imiti yimiti yimiyoboro
● Kugaragara / Ibara: Ifu yera / isobanutse Crystalline
● Gushonga Ingingo:> 300 ° C (Lit.)
Indangagaciro iroba: 1.6300 (Ikigereranyo)
● PKA: 6.8 (kuri 25 ℃)
● Zab: 131.98000
● Ubucucike: 1.505 G / CM3
● LogP: 0.41700
SHAKA TEMP.
.:0.1 m naoh: 0.25 g / ml, isobanutse, idafite ibara
● Amazi yoroheje. Arsonal mumazi.
● Xlogp3: -6.7
● Hydrogen Bond Abaterankunga kubara: 2
● Hydrogen Bond Kubara: 8
● Intebe ya Rotable Kubara: 6
● Misa nyayo: 302.06062865
Atom Atom Arem: 18
Kugorana: 397
Isuku / Ubwiza
99% * amakuru avuye kubitanga fatizo
1,4-piperazinebis (EthanesUlFonicacid) 99% * data kuva abatanga reagent
Amakuru ya hefy
● Pictogram (s):
Xi
Kode ya Hazard: xi
Imvugo: 36/37/38
● Amagambo y'umutekano: 22-24 / 25-37 / 39-26
Amadosiye ya MSDs
Ingirakamaro
Amasomo ya Chimique:Ibindi bikoresha -> buffer y'ibinyabuzima
Inseko ya kanonical:C1CN (CCN1CS (= O) (= o) o) CCS (= O) (= o) o
Ikoresha:Piperazine-n, N'-Bis- (2-ethanetilphonic acide) ultra -Pere> 99.5%) nigikorwa cyingenzi cya chimique muburyo bwose bwimiti. Acide 1,4-piperazinetaichaneskonic, N'-Bis- (2-ethaneskonic igerageza ryibinyabuzima ikoresha 6.8 kuri dodesimetero 20. Umukozi wigenga hamwe na PKA hafi ya Physiologiya Ph.pipes-N, N'-Bis (2-ethaneskonic (2-ethaneskonic (aside 2 Ni acide ya ethanesUlfonic yatejwe imbere nibyiza et al. mu myaka ya za 1960. Imiyoboro ifite PKA hafi ya Ph Ph Physiologiya Ph ituma ari ingirakamaro mumirimo yumuco seliji. Byaranditse kugirango ugabanye igihombo cya lipid iyo buffing glutaraldehyde estologiya mu bimera n'ibiti. Imiyoboro, imiyoboro ihari: Umunyu wa Sesquisoum; Imiyoboro ya dipotassiyumu; Imiyoboro yo kwikuramo umunyu; Imiyoboro, sodium umunyu. Buffers irashobora gutegurwa yongeyeho igisubizo cyimiyoboro yubusa, iganisha kuri PH ikwiye yumunyu wa monosodium na sther, umunyu wa ph. Imiyoboro, ikoreshwa mu bucuruzi bwa buffing muri Biochemie. Imiyoboro ifite Pka (6.76 kuri 25 ° C) hafi ya Physiologiya ya physiologiya ituma ingirakamaro mumirimo yumuco seliji. Porotokole yavuzwe ku ikoreshwa ry'imiyoboro yo gutandukana na Glyoxyulty RLANO muri agarose, kuclaese s1 mapping ya RNA, no muri RibonUclease yohereza protocole. Imiyoboro yakoreshejwe nka buffer muri Glutaraldehyde gukosora Ingero za Tissue., Imiyoboro yakoreshejwe muri poroteyine. Imiyoboro yasabwe gukoreshwa muri buffers kuri vitro yiga Caspase ya 3, 6, 7, na 8. Ubushakashatsi bwasohotse bwerekanaga akamaro ka pipes nka buffer idahuye nabyo nka poroteyine isaba. Imiyoboro yakoreshejwe mu muco w'akagari muri ibyo bisaba nk'ubuvugizi bwa poroteyiri wa mutant cyane muri Escheichia Coli.
Intangiriro irambuye
Imiyoboro yubusa, izwi kandi nka aside 1,4-piiperaziineiethanesFonic, ni buffer nziza igaragara ko ifite agaciro ka PKA isa na phy phsiologiya isanzwe phy phsiologiya asanzwe phy phsiologiya asanzwe Ph. Nkuko acide yubusa aside ikoreshwa kenshi nka buffer mubijyanye nubushakashatsi bwibinyabuzima.pipes ni zwrityintic, buffer ya pitriceine, ifite akamaro ka ph 1.1 - 7.5. Imiyoboro ibura ubushobozi bwo gukora ikintu gikomeye hamwe nion nyinshi kandi birasabwa gukoreshwa nkibintu bidahuza nibisubizo hamwe na feri. Imiyoboro ifite ibisobanuro bitandukanye kandi bikunze gukoreshwa mu bitangazamakuru by'umuco Iyi buffer irashobora gukora imirasire kandi rero ntabwo ikwiriye gusubiramo reaction. Birakwiriye gukoreshwa muri aside bicinchoninic (BCA). Kukeshagura imiyoboro yiyongera mugihe aside yubusa yahinduwe kuri sodium yumutima
Imiyoboro ni umunyamuryango wa acide ya EthanesUlfonic, ubanza yatangijwe na nziza et al. Kuva PKA yayo kuri 37 ° C iri hafi ya Physiologiya Ph, imiyoboro ifite ibyifuzo mumurimo wumuco wa selile.
Intangiriro irambuye
Imiyoboro, izwi kandi ku izina rya Piperazine - n, N'-Bis (2-ethaneskonic aside), ni buffer isanzwe ikoreshwa mubinyabuzima nubushakashatsi bwibinyabuzima. Ni virusi ya ZweTrionic ifasha kubungabunga ph ihamye mubisubizo byateganijwe.
Kimwe mubyiciro byibanze byimiyoboro biri murwego rwa biologiya na molecular hamwe na biochemisi, aho bikoreshwa nka buffer yo kwitegura no kubika ingero zibinyabuzima. Imiyoboro ifite agaciro ka PKA igera kuri 7.46, bituma bituma ari byiza kubungabunga ph urwego rwa 6.7 kugeza 7.9 ku bushyuhe bwa profIsiologiya (hafi ya dogere 37).
Imiyoboro ifite akamaro cyane cyane kubungabunga ph ihamye mugihe gikurikirana nkibisuku, Enzyme isuzuma, na Gel electrophoresis. Ifasha kugabanya PH impinduka zishobora kugira ingaruka kumiterere n'imikorere ya poroteyine na enzymes.
Ubundi buryo bwo gushyira mu bikorwa imiyoboro iri mu bushakashatsi bw'umuco. Bikunze gukoreshwa nka buffer kubatangaza umuco selile. Imiyoboro ifasha gukomeza PH ihoraho mu rwego rwo gukura, ni ngombwa mu mikurire n'imikurire ya selile. Itanga kandi ibihaha bihamye byo kwiga inzira ya sigari kandi ikora ubushakashatsi.
Imiyoboro nayo ikoreshwa mubuhanga butandukanye bwo gusesengura, nka chromatografiya na capillary electroresis. Ubushobozi bwayo bukabije na PH umutekano bigatuma bikwiranye muri ubwo buhanga, aho ubuyobozi bwa PH bukenewe kubisubizo nyabyo.
Byongeye kandi, imiyoboro yasanze ibyifuzo muri sisitemu yo gutanga ibiyobyabwenge na sisitemu yo gutanga. Irashobora gukoreshwa nka buffer muri farumasi yo guhitamo ituze ry'ibiyobyabwenge no kuzamura ibiyobyabwenge.
Muri rusange, imiyoboro ni umukozi uhuza amabuye atandukanye mubijyanye nubushakashatsi bwibinyabuzima nubuzima, umuco wakagari, tekinike yisesengura, hamwe nibikorwa bya farumasi. Ubushobozi bwayo bwo kubungabunga Ph ihamye ituma ari igikoresho cyingenzi mumirima itandukanye ya siyansi.
